Journal: Plant Biotechnology Journal

Article Title: Anti‐infective immunoadhesins from plants

doi: 10.1111/pbi.12441

Figure Lengend Snippet: Inhibition of cytopathic effect ( CPE ). Dilutions of sICAM ‐1, ICAM ‐1‐IgG and ICAM ‐1‐IgA2 were co‐incubated with HRV ‐39 ( MOI of 0.2) for 30 min before adding to adherent Hela‐Ohio1 cells (20 000 cells/well) in 96‐well plates. Cells were incubated for 48 h at 32 °C, then stained for viability with 0.1% crystal violet. The percentage of CPE inhibition was calculated relative to cells exposed to virus alone. Means ± standard deviations of three wells per dilution are shown.

Article Snippet: A human clinical trial funded by Boehringer Ingelheim Pharmaceuticals showed that sICAM‐1 as a nasal spray reduced the severity of experimental HRV colds and did not result in any adverse effects or evidence of absorption through the nasal mucosa (Turner et al ., ).

Techniques: Inhibition, Incubation, Staining

Journal: Pharmaceuticals

Article Title: Suramin Affects the Renal VEGF-A/VEGFR Axis in Short-Term Streptozotocin-Induced Diabetes

doi: 10.3390/ph16030470

Figure Lengend Snippet: Effects of suramin on blood concentration of soluble vascular cell adhesion molecule-1, sVCAM-1 ( A ) and soluble intercellular adhesion molecule-1, sICAM-1 ( B ) in non-diabetic and diabetic rats. Suramin (SUR, 10 mg/kg) or saline (non-SUR) were injected into non-diabetic rats (CONTROL) and streptozotocin-induced diabetic rats (DIABETES) on days 0 and +7 after streptozotocin or citrate buffer injection on day –7. The results are presented as single data points with mean values ± standard error of the mean (sVCAM-1) or as median and 25th–75th percentile (sICAM-1). Statistical significance: * p = 0.0001 vs. control non-SUR (two-way ANOVA).

Article Snippet: Immunoenzymatic assays were used to measure the concentration of rat sVCAM-1 (MyBioSource, San Diego, CA, USA, Cat. No. MBS762680) and rat sICAM-1 (MyBioSource, San Diego, CA, USA, Cat. No. MBS266128).

Techniques: Concentration Assay, Injection